ARG62896
anti-CD58 antibody [MEM-63] (Biotin)
anti-CD58 antibody [MEM-63] (Biotin) for Flow cytometry and Human,Pig
Immune System antibody
Overview
| Product Description | Biotin-conjugated Mouse Monoclonal antibody [MEM-63] recognizes CD58 |
|---|---|
| Tested Reactivity | Hu, Pig |
| Tested Application | FACS |
| Specificity | The clone MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts. HLDA VI; WS Code AS A047 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-63 |
| Isotype | IgG1 |
| Target Name | CD58 |
| Antigen Species | Human |
| Immunogen | NALM-6 human pre-B cell line |
| Conjugation | Biotin |
| Alternate Names | CD antigen CD58; LFA3; ag3; Lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3; Ag3; LFA-3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P19256 Human Lymphocyte function-associated antigen 3 |
|---|---|
| Gene Symbol | CD58 |
| Gene Full Name | CD58 molecule |
| Background | CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B. |
| Function | Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 28 kDa |
