ARG53881

anti-CD59 antibody [MEM-43] (APC)

anti-CD59 antibody [MEM-43] (APC) for Flow cytometry and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description

APC-conjugated Mouse Monoclonal antibody [MEM-43] recognizes CD59

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.
HLDA IV; WS Code NL 705
HLDA V; WS Code AS S013
HLDA V; WS Code BP BP345
HLDA V; WS Code T T-103
Host Mouse
Clonality Monoclonal
Clone MEM-43
Isotype IgG2a
Target Name CD59
Immunogen Thymocytes and T lymphocytes
Conjugation APC
Alternate Names EJ30; MIRL; Membrane attack complex inhibition factor; CD antigen CD59; EJ16; Membrane inhibitor of reactive lysis; MIC11; EL32; HRF20; HRF-20; MEM43 antigen; MIN1; MIN2; MIN3; 1F5 antigen; 1F5; MACIF; MAC-IP; MSK21; Protectin; G344; p18-20; CD59 glycoprotein; MEM43; MAC-inhibitory protein; 16.3A5; 20 kDa homologous restriction factor

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 966 Human CD59

Swiss-port # P13987 Human CD59 glycoprotein

Gene Symbol CD59
Gene Full Name CD59 molecule, complement regulatory protein
Background CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Function Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 14 kDa
PTM N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.

Clone References

6-o- and N-sulfated syndecan-1 promotes baculovirus binding and entry into Mammalian cells.

Makkonen KE et al.
J Virol.,  (2013)

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The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction.

WB, ICC/IF / Mouse

Dun MD et al.
J Biol Chem.,  (2011)

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hr_line

Mapping the intermedilysin-human CD59 receptor interface reveals a deep correspondence with the binding site on CD59 for complement binding proteins C8alpha and C9.

FuncSt / Human

Wickham SE et al.
J Biol Chem.,  (2011)

publication_link

 

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Differential transformation capacity of Src family kinases during the initiation of prostate cancer.

WB / Human

Cai H et al.
Proc Natl Acad Sci U S A.,  (2011)

publication_link

 

hr_line

The complement inhibitors Crry and factor H are critical for preventing autologous complement activation on renal tubular epithelial cells.

FACS / 

Renner B et al.
J Immunol.,  (2010)

publication_link

 

hr_line

Oxidative stress and the regulation of complement activation in human glaucoma.

IHC-P / Human

Tezel G et al.
Invest Ophthalmol Vis Sci.,  (2010)

publication_link

 

hr_line

Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration.

FACS / Human

Kolev MV et al.
Pharmacogenomics J.,  (2010)

publication_link

 

hr_line

Basolateral internalization of GPI-anchored proteins occurs via a clathrin-independent flotillin-dependent pathway in polarized hepatic cells.

ICC/IF / Human

Aït-Slimane T et al.
Mol Biol Cell.,  (2009)

publication_link

 

hr_line

The binding of factor H to a complex of physiological polyanions and C3b on cells is impaired in atypical hemolytic uremic syndrome.

ELISA / Human

Ferreira VP et al.
J Immunol.,  (2009)

publication_link

 

hr_line

Intermedilysin-receptor interactions during assembly of the pore complex: assembly intermediates increase host cell susceptibility to complement-mediated lysis.

FRET / Human

LaChapelle S et al.
J Biol Chem.,  (2009)

publication_link

 

hr_line

Salivary proteomics for oral cancer biomarker discovery.

WB / Human

Hu S et al.
Clin Cancer Res.,  (2008)

publication_link

 

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