ARG20931
anti-CD61 / Integrin beta 3 antibody [PM6/13]
anti-CD61 / Integrin beta 3 antibody [PM6/13] for Blocking,ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [PM6/13] recognizes CD61 / Integrin beta 3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | BL, ELISA, FACS, ICC/IF, IP, WB |
| Specificity | Human CD61. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PM6/13 |
| Isotype | IgG1, kappa |
| Target Name | CD61 / Integrin beta 3 |
| Antigen Species | Human |
| Immunogen | Human platelet plasma membrane |
| Conjugation | Un-conjugated |
| Alternate Names | GT; CD antigen CD61; CD61; BDPLT2; GPIIIa; BDPLT16; GP3A; Platelet membrane glycoprotein IIIa; Integrin beta-3 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | BBS (pH 8.2) |
| Concentration | 0.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGB3 |
| Gene Full Name | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
| Background | The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008] |
| Function | Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt] |
| Calculated MW | 87 kDa |
| PTM | Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. |
