ARG62905

anti-CD62L / L-Selectin antibody [DREG56]

anti-CD62L / L-Selectin antibody [DREG56] for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [DREG56] recognizes CD62L / L-Selectin
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, FuncSt, IHC-Fr, IP, WB
Specificity The clone DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
HLDA V; WS Code S056
Host Mouse
Clonality Monoclonal
Clone DREG56
Isotype IgG1
Target Name CD62L / L-Selectin
Antigen Species Human
Immunogen PMA-activated human peripheral blood leukocytes
Conjugation Un-conjugated
Alternate Names Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS2 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
Functional studies: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6402 Human SELL

Swiss-port # P14151 Human L-selectin

Gene Symbol SELL
Gene Full Name selectin L
Background CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
Function Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. [UniProt]
Highlight Related products:
CD62L antibodies; CD62L ELISA Kits; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 42 kDa

Clone References

In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin.

Killock DJ et al.
J Biol Chem.,  (2009)

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Critical role of Lck in L-selectin signaling induced by sulfatides engagement.

Xu T et al.
J Leukoc Biol.,  (2008)

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L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.

Jutila MA et al.
J Immunol.,  (2002)

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Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep.

Abraham WM et al.
Am J Respir Crit Care Med.,  (1999)

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