ARG62907

anti-CD62L / L-Selectin antibody [DREG56] (FITC)

anti-CD62L / L-Selectin antibody [DREG56] (FITC) for Flow cytometry and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [DREG56] recognizes CD62L / L-Selectin
Tested Reactivity Hu
Tested Application FACS
Specificity The clone DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
HLDA V; WS Code S056
Host Mouse
Clonality Monoclonal
Clone DREG56
Isotype IgG1
Target Name CD62L / L-Selectin
Antigen Species Human
Immunogen PMA-activated human peripheral blood leukocytes
Conjugation FITC
Alternate Names Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6402 Human SELL

Swiss-port # P14151 Human L-selectin

Gene Symbol SELL
Gene Full Name selectin L
Background CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
Function Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 42 kDa

Clone References

In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin.

Killock DJ et al.
J Biol Chem.,  (2009)

publication_link

 

hr_line

Critical role of Lck in L-selectin signaling induced by sulfatides engagement.

Xu T et al.
J Leukoc Biol.,  (2008)

publication_link

 

hr_line

L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.

Jutila MA et al.
J Immunol.,  (2002)

publication_link

 

hr_line

Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep.

Abraham WM et al.
Am J Respir Crit Care Med.,  (1999)

publication_link

 

hr_line