ARG42260

anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin)

anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [DREG56] recognizes CD62L / L-Selectin
Tested Reactivity Hu
Tested Application FACS, FuncSt, IHC-Fr, IP, WB
Specificity The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
Host Mouse
Clonality Monoclonal
Clone DREG56
Isotype IgG1
Target Name CD62L / L-Selectin
Antigen Species Human
Immunogen PMA-activated Human peripheral blood leukocytes.
Conjugation Un-conjugated
Alternate Names Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein.
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6402 Human SELL

Swiss-port # P14151 Human L-selectin

Gene Symbol SELL
Gene Full Name selectin L
Background This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
Function Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28489325, PubMed:28011641). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 42 kDa