ARG62908

anti-CD62L / L-Selectin antibody [IVA94]

anti-CD62L / L-Selectin antibody [IVA94] for Flow cytometry,ICC/IF,Immunoprecipitation and Bovine

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [IVA94] recognizes CD62L / L-Selectin
Tested Reactivity Bov
Tested Application FACS, ICC/IF, IP
Specificity The clone IVA94 reacts with CD62L antigen (bovine). CD62L (L-selectin) is a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
Host Mouse
Clonality Monoclonal
Clone IVA94
Isotype IgG1
Target Name CD62L / L-Selectin
Immunogen Bovine leukocytes.
Conjugation Un-conjugated
Alternate Names Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 281485 Bovine SELL

Swiss-port # P98131 Bovine L-selectin

Gene Symbol SELL
Gene Full Name selectin L
Background This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
Function Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 42 kDa

Clone References

Nomenclature and characterization of leukocyte differentiation antigens in ruminants.

Naessens J et al.
Immunol Today.,  (1997)

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Biochemical analysis of preliminary clusters in the non-lineage panel.

Naessens J et al.
Vet Immunol Immunopathol.,  (1996)

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Ruminant cluster CD62L.

Howard CJ et al.
Vet Immunol Immunopathol.,  (1996)

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Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995.

[No authors listed] et al.
Vet Immunol Immunopathol.,  (1996)

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