ARG62909

anti-CD62L / L-Selectin antibody [MEL-14] (FITC)

anti-CD62L / L-Selectin antibody [MEL-14] (FITC) for Blocking,Flow cytometry,IHC-Frozen sections and Mouse

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description FITC-conjugated Rat Monoclonal antibody [MEL-14] recognizes CD62L / L-Selectin
Tested Reactivity Ms
Tested Application BL, FACS, IHC-Fr
Host Rat
Clonality Monoclonal
Clone MEL-14
Isotype IgG2a, kappa
Target Name CD62L / L-Selectin
Antigen Species Mouse
Immunogen C3H/eb cloned mouse B cell lymphoma 38C-13
Conjugation FITC
Alternate Names Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 2 µg/10^6 cells
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20343 Mouse SELL

Swiss-port # P18337 Mouse L-selectin

Gene Symbol Sell
Gene Full Name selectin, lymphocyte
Background CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). _x000D_
Function Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 42 kDa

Clone References

Coadministration of polyinosinic:polycytidylic acid and immunostimulatory complexes modifies antigen processing in dendritic cell subsets and enhances HIV gag-specific T cell immunity.

ICC/IF / 

Quinn KM et al.
J Immunol.,  (2013)

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Comparative analysis of the magnitude, quality, phenotype, and protective capacity of simian immunodeficiency virus gag-specific CD8+ T cells following human-, simian-, and chimpanzee-derived recombinant adenoviral vector immunization.

Quinn KM et al.
J Immunol.,  (2013)

publication_link

 

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IL-10 restricts memory T cell inflation during cytomegalovirus infection.

FACS / Mouse

Jones M et al.
J Immunol.,  (2010)

publication_link

 

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TLR-mediated loss of CD62L focuses B cell traffic to the spleen during Salmonella typhimurium infection.

FACS / Mouse

Morrison VL et al.
J Immunol.,  (2010)

publication_link

 

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Distribution of MEL-14+ cells in various lymphoid tissues.

FACS, FuncSt / Mouse

Iwabuchi K et al.
Immunobiology.,  (1991)

publication_link

 

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