ARG65485
anti-CD62L / L-Selectin antibody [MEL-14] (low endotoxin)
anti-CD62L / L-Selectin antibody [MEL-14] (low endotoxin) for Flow cytometry,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ICC/IF,Functional study and Mouse
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Azide free and low endotoxin Rat Monoclonal antibody [MEL-14] recognizes CD62L / L-Selectin |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP |
| Specificity | The clone MEL-14 reacts with mouse CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells._x000D_ |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | MEL-14 |
| Isotype | IgG2a |
| Target Name | CD62L / L-Selectin |
| Antigen Species | Mouse |
| Immunogen | C3H/eb mouse B cell lymphoma 38C-13_x000D_ |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: Blocking of cell adhesion. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Sell |
| Gene Full Name | selectin, lymphocyte |
| Background | CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). _x000D_ |
| Function | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 42 kDa |
Clone References
Coadministration of polyinosinic:polycytidylic acid and immunostimulatory complexes modifies antigen processing in dendritic cell subsets and enhances HIV gag-specific T cell immunity.
ICC/IF /
Comparative analysis of the magnitude, quality, phenotype, and protective capacity of simian immunodeficiency virus gag-specific CD8+ T cells following human-, simian-, and chimpanzee-derived recombinant adenoviral vector immunization.
IL-10 restricts memory T cell inflation during cytomegalovirus infection.
FACS / Mouse
TLR-mediated loss of CD62L focuses B cell traffic to the spleen during Salmonella typhimurium infection.
FACS / Mouse
