ARG65485

anti-CD62L / L-Selectin antibody [MEL-14] (low endotoxin)

anti-CD62L / L-Selectin antibody [MEL-14] (low endotoxin) for Flow cytometry,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ICC/IF,Functional study and Mouse

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [MEL-14] recognizes CD62L / L-Selectin
Tested Reactivity Ms
Tested Application FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP
Specificity The clone MEL-14 reacts with mouse CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells._x000D_
Host Rat
Clonality Monoclonal
Clone MEL-14
Isotype IgG2a
Target Name CD62L / L-Selectin
Antigen Species Mouse
Immunogen C3H/eb mouse B cell lymphoma 38C-13_x000D_
Conjugation Un-conjugated
Alternate Names Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note Functional studies: Blocking of cell adhesion.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20343 Mouse SELL

Swiss-port # P18337 Mouse L-selectin

Gene Symbol Sell
Gene Full Name selectin, lymphocyte
Background CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). _x000D_
Function Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 42 kDa

Clone References

Coadministration of polyinosinic:polycytidylic acid and immunostimulatory complexes modifies antigen processing in dendritic cell subsets and enhances HIV gag-specific T cell immunity.

ICC/IF / 

Quinn KM et al.
J Immunol.,  (2013)

publication_link

 

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Comparative analysis of the magnitude, quality, phenotype, and protective capacity of simian immunodeficiency virus gag-specific CD8+ T cells following human-, simian-, and chimpanzee-derived recombinant adenoviral vector immunization.

Quinn KM et al.
J Immunol.,  (2013)

publication_link

 

hr_line

IL-10 restricts memory T cell inflation during cytomegalovirus infection.

FACS / Mouse

Jones M et al.
J Immunol.,  (2010)

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hr_line

TLR-mediated loss of CD62L focuses B cell traffic to the spleen during Salmonella typhimurium infection.

FACS / Mouse

Morrison VL et al.
J Immunol.,  (2010)

publication_link

 

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