ARG23104

anti-CD62P / P-Selectin antibody [AK-6] (azide free)

anti-CD62P / P-Selectin antibody [AK-6] (azide free) for IHC-Frozen sections,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rhesus Monkey

Overview

Product Description Azide free Mouse Monoclonal antibody [AK-6] recognizes CD62P / P-Selectin
Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.CD62P is also known as P-selectin, PADGEM or GMP140 and plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the alpha granule and is translocated to the plasma membrane upon activation.
Tested Reactivity Hu, R. Mk
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone AK-6
Isotype IgG1
Target Name CD62P / P-Selectin
Antigen Species Human
Immunogen Human platelet membrane glycoproteins.
Conjugation Un-conjugated
Alternate Names PADGEM; CD62; Platelet activation dependent granule-external membrane protein; CD62 antigen-like family member P; GMP140; PSEL; Granule membrane protein 140; CD62P; CD antigen CD62P; Leukocyte-endothelial cell adhesion molecule 3; GRMP; GMP-140; P-selectin; LECAM3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:100
ICC/IFAssay-dependent
IHC-Fr1:1000 - 1:5000
IHC-P1:500 - 1:2000
WBAssay-dependent
Application Note IHC-P: Staining may be enhanced using Sodium Citrate buffer pH 6.0
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6403 Human SELP

Swiss-port # P16109 Human P-selectin

Gene Symbol SELP
Gene Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Background This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]
Function Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. [UniProt]
Calculated MW 91 kDa