ARG54215

anti-CD62P / P-Selectin antibody [AK4] (APC)

anti-CD62P / P-Selectin antibody [AK4] (APC) for Flow cytometry and Human,Primates

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [AK4] recognizes CD62P / P-Selectin
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
Workshop: HLDA VI: WS Code P-44.
Host Mouse
Clonality Monoclonal
Clone AK4
Isotype IgG1
Target Name CD62P / P-Selectin
Antigen Species Human
Immunogen Human platelets
Conjugation APC
Alternate Names PADGEM; CD62; Platelet activation dependent granule-external membrane protein; CD62 antigen-like family member P; GMP140; PSEL; Granule membrane protein 140; CD62P; CD antigen CD62P; Leukocyte-endothelial cell adhesion molecule 3; GRMP; GMP-140; P-selectin; LECAM3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6403 Human SELP

Swiss-port # P16109 Human P-selectin

Gene Symbol SELP
Gene Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Background CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
Function Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 91 kDa

Clone References

Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules.

Ludwig RJ et al.
J Invest Dermatol.,  (2004)

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GMP-140 binding to neutrophils is inhibited by sulfated glycans.

Skinner MP et al.
J Biol Chem.,  (1991)

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Characterization of GMP-140 (P-selectin) as a circulating plasma protein.

Dunlop LC et al.
J Exp Med.,  (1992)

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