ARG65407
anti-CD62P / P-Selectin antibody [AK4]
anti-CD62P / P-Selectin antibody [AK4] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,Immunoprecipitation,Western blot and Human,Primates
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Overview
Product Description | Mouse Monoclonal antibody [AK4] recognizes CD62P / P-Selectin |
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Tested Reactivity | Hu, NHuPrm |
Tested Application | CyTOF®-candidate, FACS, FuncSt, ICC/IF, IP, WB |
Specificity | The clone AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
Workshop: HLDA VI: WS Code P-44 |
Host | Mouse |
Clonality | Monoclonal |
Clone | AK4 |
Isotype | IgG1 |
Target Name | CD62P / P-Selectin |
Antigen Species | Human |
Immunogen | Human platelets |
Conjugation | Un-conjugated |
Alternate Names | PADGEM; CD62; Platelet activation dependent granule-external membrane protein; CD62 antigen-like family member P; GMP140; PSEL; Granule membrane protein 140; CD62P; CD antigen CD62P; Leukocyte-endothelial cell adhesion molecule 3; GRMP; GMP-140; P-selectin; LECAM3 |
Application Instructions
Application Suggestion |
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Application Note | Functional studies: Blocking. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from ascites by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
Preservative | 15 mM Sodium azide |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | SELP |
Gene Full Name | selectin P (granule membrane protein 140kDa, antigen CD62) |
Background | CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster. |
Function | Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. [UniProt] |
Highlight | Related products: CD62P antibodies; CD62P ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody |
Calculated MW | 91 kDa |