ARG65361

anti-CD64 antibody [10.1]

anti-CD64 antibody [10.1] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Primates

Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [10.1] recognizes CD64

Tested Reactivity Hu, NHuPrm
Tested Application CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The clone 10.1 recognizes alpha subunit of CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.
HLDA III; WS Code M-250
Host Mouse
Clonality Monoclonal
Clone 10.1
Isotype IgG1
Target Name CD64
Antigen Species Human
Immunogen Rheumatoid synovial fluid cells and fibronectin purified human monocytes
Conjugation Un-conjugated
Alternate Names High affinity immunoglobulin gamma Fc receptor I; CD64; Fc-gamma RIA; CD antigen CD64; FcgammaRIa; FCRI; IgG Fc receptor I; CD64A; Fc-gamma RI; FcRI; IGFR1

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 5 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note IHC-P and IHC-Fr: There can occur problems with paraformaldehyde fixation
Functional studies: blocking of IgG binding to the FcgammaR1.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2209 Human FCGR1A

Swiss-port # P12314 Human High affinity immunoglobulin gamma Fc receptor I

Gene Symbol FCGR1A
Gene Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Background CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
Function High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. [UniProt]
Highlight Related products:
CD64 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Immune System antibody
Calculated MW 43 kDa
PTM Phosphorylated on serine residues.

Clone References

Rap1 activation is required for Fc gamma receptor-dependent phagocytosis.

FACS / 

Chung J et al.
J Immunol.,  (2008)

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CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells.

Sánchez-Torres C et al.
Int Immunol.,  (2001)

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Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.

Yoshino N et al.
Exp Anim.,  (2000)

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High affinity IgG binding by FcgammaRI (CD64) is modulated by two distinct IgSF domains and the transmembrane domain of the receptor.

Harrison PT et al.
Protein Eng.,  (1998)

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