ARG55945

anti-CD66e / CEA antibody [COL-1]

anti-CD66e / CEA antibody [COL-1] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [COL-1] recognizes CD66e / CEA
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone COL-1
Isotype IgG2a, kappa
Target Name CD66e / CEA
Antigen Species Human
Immunogen Human colon carcinoma extract.
Conjugation Un-conjugated
Alternate Names Carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; CD antigen CD66e; Carcinoembryonic antigen; Meconium antigen 100

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1048 Human CEACAM5

Swiss-port # P06731 Human Carcinoembryonic antigen-related cell adhesion molecule 5

Gene Symbol CEACAM5
Gene Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Background This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Function Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. [UniProt]
Cellular Localization Cytoplasmic and luminal surface
Calculated MW 77 kDa
PTM Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.