ARG23387

anti-CD68 antibody [ED1]

anti-CD68 antibody [ED1] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Radioimmunoassay,Western blot and Bovine,Mouse,Rat

Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
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Overview

Product Description

Mouse Monoclonal antibody [ED1] recognizes CD68
Mouse anti rat CD68, clone ED1 recognizes the rat ED1 antigen. The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat.

Tested Reactivity Ms, Rat, Bov
Species Does Not React With Hrs
Tested Application FACS, IHC-Fr, IHC-P, IP, RIA, WB
Host Mouse
Clonality Monoclonal
Clone ED1
Isotype IgG1
Target Name CD68
Antigen Species Rat
Immunogen Rat spleen cells
Conjugation Un-conjugated
Alternate Names Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:10
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
RIAAssay-dependent
WBAssay-dependent
Application Note FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
IHC-P: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12514 Mouse CD68

Swiss-port # P31996 Mouse Macrosialin

Gene Symbol CD68
Gene Full Name CD68 molecule
Background CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Function CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Highlight Related products:
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Research Area Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
Calculated MW 37 kDa
PTM N- and O-glycosylated. [UniProt]

Customer's Feedback

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Review for anti-CD68 antibody [ED1]

Application:WB

Sample:Rat spleen

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Preparation and renoprotective effects of carboxymethyl chitosan oligosaccharide on adriamycin nephropathy

IHC-P / Rat

Qiao Jing et al.
Carbohydrate Polymers.,  (2018)

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