ARG23069

anti-CD68 antibody [KP1] (FITC)

anti-CD68 antibody [KP1] (FITC) for Flow cytometry,ICC/IF and Human,Primates,Rat

Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
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Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [KP1] recognizes CD68
Mouse anti human CD68 antibody, clone KP1 recognises the human CD68 cell surface antigen, a 110kD glycoprotein primarily expressed by macrophages and monocytes.
Tested Reactivity Hu, Rat, NHuPrm
Tested Application FACS, ICC/IF
Host Mouse
Clonality Monoclonal
Clone KP1
Isotype IgG1
Target Name CD68
Antigen Species Human
Immunogen Subcellular fraction of Human alveolar macrophages
Conjugation FITC
Alternate Names Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:10
ICC/IFAssay-dependent
Application Note FACS: Membrane permeabilization is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide and 1% BSA
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 968 Human CD68

Swiss-port # P34810 Human Macrosialin

Gene Symbol CD68
Gene Full Name CD68 molecule
Background CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Function CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
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Research Area Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
Calculated MW 37 kDa
PTM N- and O-glycosylated.

Specific References

SOCS2 Suppresses Inflammation and Apoptosis during NASH Progression through Limiting NF-κB Activation in Macrophages

FACS / Human

Shuo Li et al.
Int J Biol Sci.,  (2021)

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PTPROt Aggravates Inflammation by Enhancing NF-κB Activation in Liver Macrophages During Nonalcoholic Steatohepatitis.

FACS / Human

Jin Kangpeng et al.
Theranostics.,  (2020)

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