ARG52803

anti-CD68 antibody [SP251]

anti-CD68 antibody [SP251] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human

Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody

Overview

Product Description

Rabbit Monoclonal antibody [SP251] recognizes CD68

Tested Reactivity Hu
Tested Application FACS, IHC-P
Host Rabbit
Clonality Monoclonal
Clone SP251
Isotype IgG
Target Name CD68
Antigen Species Human
Immunogen Synthetic peptide derived from the internal region of the human CD68 protein.
Conjugation Un-conjugated
Alternate Names Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
IHC-P1:100
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Tonsil

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 968 Human CD68

Swiss-port # P34810 Human Macrosialin

Background CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Function CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Cellular Localization Cytoplasm
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Research Area Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
Calculated MW 37 kDa
PTM N- and O-glycosylated.