ARG42327

anti-CD68 antibody [Y1/82A] (APC)

anti-CD68 antibody [Y1/82A] (APC) for Flow cytometry and Human

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [Y1/82A] recognizes CD68
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody Y1/82A recognizes CD68 (LAMP4), a 110 kDa glycoprotein expressed mainly in cytoplasmic granules of monocytes/macrophages, granulocytes, and dendritic cells.
Host Mouse
Clonality Monoclonal
Clone Y1/82A
Isotype IgG2a
Target Name CD68
Antigen Species Human
Immunogen Lysosomal contents of lung macrophages.
Conjugation APC
Alternate Names Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 968 Human CD68

Swiss-port # P34810 Human Macrosialin

Gene Symbol CD68
Gene Full Name CD68 molecule
Background This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Cellular Localization Isoform Short: Cell membrane; Single-pass type I membrane protein. Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. [UniProt]
Highlight Related products:
CD68 antibodies; CD68 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Calculated MW 37 kDa
PTM N- and O-glycosylated. [UniProt]