ARG62916

anti-CD69 antibody [FN50]

anti-CD69 antibody [FN50] for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [FN50] recognizes CD69

Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, FuncSt, IHC-Fr
Specificity The clone FN50 recognizes CD69, an lymphocyte early activation marker.
HLDA IV; WS Code A 91
Host Mouse
Clonality Monoclonal
Clone FN50
Isotype IgG1
Target Name CD69
Antigen Species Human
Immunogen anti-µ-stimulated human B lymphocytes
Conjugation Un-conjugated
Alternate Names GP32/28; Activation inducer molecule; MLR-3; BL-AC/P26; Leukocyte surface antigen Leu-23; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; EA1; CD antigen CD69; CLEC2C; C-type lectin domain family 2 member C

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
Application Note Functional studies: When stimulating peripheral blood T cells, clone FN50 together with TPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 to stimulated peripheral blood B cells has similar, but weaker effect.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 969 Human CD69

Swiss-port # Q07108 Human Early activation antigen CD69

Gene Symbol CD69
Gene Full Name CD69 molecule
Background CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.
Function Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. [UniProt]
Highlight Related products:
CD69 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 23 kDa
PTM Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

Clone References

CD98hc (SLC3A2) interaction with beta 1 integrins is required for transformation.

Henderson NC et al.
J Biol Chem.,  (2004)

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Multiple dimeric forms of human CD69 result from differential addition of N-glycans to typical (Asn-X-Ser/Thr) and atypical (Asn-X-cys) glycosylation motifs.

Vance BA et al.
J Biol Chem.,  (1997)

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