ARG66810

anti-CD7 antibody [SQab20223]

anti-CD7 antibody [SQab20223] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

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Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab20223] recognizes CD7
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab20223
Isotype IgG
Target Name CD7
Antigen Species Human
Immunogen Human CD7.
Conjugation Un-conjugated
Alternate Names TP41; Tp40; T-cell antigen CD7; CD antigen CD7; T-cell leukemia antigen; LEU-9; T-cell surface antigen Leu-9; GP40

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:3000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 40 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 924 Human CD7

Swiss-port # P09564 Human T-cell antigen CD7

Gene Symbol CD7
Gene Full Name CD7 molecule
Background This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq, Jul 2008]
Function Not yet known. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 25 kDa

Customer's Feedback

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Review for anti-CD7 antibody [SQab20223]

Application:WB

Sample:JurKat

Sample Loading Amount:20 μl

SDS Page Type:Denatured

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP