ARG56159
anti-CD71 / Transferrin Receptor antibody [DF1513]
anti-CD71 / Transferrin Receptor antibody [DF1513] for Flow cytometry,ICC/IF,Western blot and Human
2
Overview
Product Description | Mouse Monoclonal antibody [DF1513] recognizes CD71 / Transferrin Receptor |
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Tested Reactivity | Hu |
Tested Application | FACS, ICC/IF, WB |
Host | Mouse |
Clonality | Monoclonal |
Clone | DF1513 |
Isotype | IgG1, kappa |
Target Name | CD71 / Transferrin Receptor |
Antigen Species | Human |
Immunogen | KG1 acute myeloid leukemia cell line. |
Conjugation | Un-conjugated |
Alternate Names | TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purification with Protein G. |
Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
Preservative | 0.05% Sodium azide |
Stabilizer | 0.1 mg/ml BSA |
Concentration | 0.2 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | TFRC |
Gene Full Name | transferrin receptor |
Background | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] |
Function | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt] |
Calculated MW | ~ 85 - 95 kDa |
PTM | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. |
Customer's Feedback
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Review for anti-CD71 / Transferrin Receptor antibody [DF1513]
Application:IF/ICC
Sample:HeLa
Fixation Buffer:100% Methanol
Fixation Time:10 min
Fixation Temperature:RT ºC
Permeabilization Buffer:0.1% Triton X-100
Primary Antibody Dilution Factor:1:100
Primary Antibody Incubation Time:overnight
Primary Antibody Incubation Temperature:4 ºC
Conjugation of Secondary Antibody:FITC