ARG56159

anti-CD71 / Transferrin Receptor antibody [DF1513]

anti-CD71 / Transferrin Receptor antibody [DF1513] for Flow cytometry,ICC/IF,Western blot and Human

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Overview

Product Description Mouse Monoclonal antibody [DF1513] recognizes CD71 / Transferrin Receptor
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone DF1513
Isotype IgG1, kappa
Target Name CD71 / Transferrin Receptor
Antigen Species Human
Immunogen KG1 acute myeloid leukemia cell line.
Conjugation Un-conjugated
Alternate Names TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 2 µg/ml
WB0.25 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7037 Human TFRC

Swiss-port # P02786 Human Transferrin receptor protein 1

Gene Symbol TFRC
Gene Full Name transferrin receptor
Background This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt]
Calculated MW ~ 85 - 95 kDa
PTM N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Customer's Feedback

nuts_pic      Good

Review for anti-CD71 / Transferrin Receptor antibody [DF1513]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-CD71 / Transferrin Receptor antibody [DF1513]

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:0.25 μg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC