ARG62922

anti-CD71 / Transferrin Receptor antibody [MEM-189]

anti-CD71 / Transferrin Receptor antibody [MEM-189] for Flow cytometry,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-189] recognizes CD71 / Transferrin Receptor
Tested Reactivity Hu
Tested Application FACS, IP, WB
Specificity The clone MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.
MEM-189 does not block binding of transferrin to the receptor.
HLDA VI; WS Code NL N-L025
Host Mouse
Clonality Monoclonal
Clone MEM-189
Isotype IgG1
Target Name CD71 / Transferrin Receptor
Antigen Species Human
Immunogen KG1 human acute myelogenous leukaemia cell line
Conjugation Un-conjugated
Alternate Names TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer TBS (pH 8.0) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7037 Human TFRC

Swiss-port # P02786 Human Transferrin receptor protein 1

Gene Symbol TFRC
Gene Full Name transferrin receptor
Background CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody
Calculated MW 85 kDa
PTM N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Clone References

Aptamers as potential tools for super-resolution microscopy.

Opazo F et al.
Nat Methods.,  (2012)

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Glucocorticoid treatment skews human monocyte differentiation into a hemoglobin-clearance phenotype with enhanced heme-iron recycling and antioxidant capacity.

WB / Human

Vallelian F et al.
Blood.,  (2010)

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Highly multiparametric analysis by mass cytometry.

FACS / Human

Ornatsky O et al.
J Immunol Methods.,  (2010)

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Cell entry of the aphthovirus equine rhinitis A virus is dependent on endosome acidification.

ICC/IF / Human

Groppelli E et al.
J Virol.,  (2010)

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Mesenchymal stem cells as a gene therapy carrier for treatment of fibrosarcoma.

ICC/IF / Rat

Xiang J et al.
Cytotherapy.,  (2009)

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CD1a and MHC class I follow a similar endocytic recycling pathway.

IP / Human

Barral DC et al.
Traffic.,  (2008)

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