ARG20942
anti-CD71 / Transferrin Receptor antibody [RI7217]
anti-CD71 / Transferrin Receptor antibody [RI7217] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot,Blocking and Mouse
Overview
Product Description | Rat Monoclonal antibody [RI7217] recognizes CD71 / Transferrin Receptor |
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Tested Reactivity | Ms |
Tested Application | BL, FACS, ICC/IF, IP, WB |
Specificity | Mouse CD71. The clone RI7217 inhibits cell proliferation in vitro. |
Host | Rat |
Clonality | Monoclonal |
Clone | RI7217 |
Isotype | IgG2a, kappa |
Target Name | CD71 / Transferrin Receptor |
Antigen Species | Mouse |
Immunogen | DMSO induced Friend erythroleukemia 745.6 |
Conjugation | Un-conjugated |
Alternate Names | TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Buffer | BBS (pH 8.2) |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | TFRC |
Gene Full Name | transferrin receptor |
Background | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] |
Function | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt] |
Calculated MW | 85 kDa |
PTM | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. |