ARG53897
anti-CD72 antibody [3F3] (PE)
anti-CD72 antibody [3F3] (PE) for Flow cytometry and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [3F3] recognizes CD72 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The clone 3F3 reacts with CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells. HLDA V; WS Code B CD72.5 HLDA VI; WS Code B CD72.1 HLDA VI; WS Code 6 BP 84 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3F3 |
| Isotype | IgG2b |
| Target Name | CD72 |
| Antigen Species | Human |
| Immunogen | Normal human lymphocytes from a lymph node. |
| Conjugation | PE |
| Alternate Names | CD antigen CD72; LYB2; B-cell differentiation antigen CD72; Lyb-2; CD72b |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P21854 Human B-cell differentiation antigen CD72 |
|---|---|
| Gene Symbol | CD72 |
| Gene Full Name | CD72 molecule |
| Background | CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation. |
| Function | Plays a role in B-cell proliferation and differentiation. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 40 kDa |
| PTM | Phosphorylated upon engagement of the B-cell receptor, probably by LYN or SYK. Phosphorylation at Tyr-7 is important for interaction with PTPN6/SHP-1 (By similarity). |
