ARG53897

anti-CD72 antibody [3F3] (PE)

anti-CD72 antibody [3F3] (PE) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description

PE-conjugated Mouse Monoclonal antibody [3F3] recognizes CD72

Tested Reactivity Hu
Tested Application FACS
Specificity The clone 3F3 reacts with CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.
HLDA V; WS Code B CD72.5
HLDA VI; WS Code B CD72.1
HLDA VI; WS Code 6 BP 84
Host Mouse
Clonality Monoclonal
Clone 3F3
Isotype IgG2b
Target Name CD72
Antigen Species Human
Immunogen Normal human lymphocytes from a lymph node.
Conjugation PE
Alternate Names CD antigen CD72; LYB2; B-cell differentiation antigen CD72; Lyb-2; CD72b

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 971 Human CD72

Swiss-port # P21854 Human B-cell differentiation antigen CD72

Gene Symbol CD72
Gene Full Name CD72 molecule
Background CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.
Function Plays a role in B-cell proliferation and differentiation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 40 kDa
PTM Phosphorylated upon engagement of the B-cell receptor, probably by LYN or SYK. Phosphorylation at Tyr-7 is important for interaction with PTPN6/SHP-1 (By similarity).