ARG42312

anti-CD73 antibody [AD2] (APC)

anti-CD73 antibody [AD2] (APC) for Flow cytometry and Human

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [AD2] recognizes CD73
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.
Host Mouse
Clonality Monoclonal
Clone AD2
Isotype IgG1, kappa
Target Name CD73
Antigen Species Human
Immunogen Human CD73.
Conjugation APC
Alternate Names 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4907 Human NT5E

Swiss-port # P21589 Human 5'-nucleotidase

Gene Symbol NT5E
Gene Full Name 5'-nucleotidase, ecto (CD73)
Background The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
Calculated MW 63 kDa