ARG55075

anti-CD73 antibody

anti-CD73 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody

Overview

Product Description Mouse Monoclonal antibody recognizes CD73
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 974CT7.5.3
Isotype IgG1
Target Name CD73
Antigen Species Human
Immunogen Purified His-tagged Human CD73 protein.
Conjugation Un-conjugated
Alternate Names 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:25
WB1:5000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WiDr

Properties

Form Liquid
Purification Purification with Protein G.
Buffer Crude Ascites and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4907 Human NT5E

Swiss-port # P21589 Human 5'-nucleotidase

Gene Symbol NT5E
Gene Full Name 5'-nucleotidase, ecto (CD73)
Background The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor.
Research Area Cell Biology and Cellular Response antibody
Calculated MW 63 kDa