ARG22257

anti-CD74 antibody [PIN.1]

anti-CD74 antibody [PIN.1] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation,Flow cytometry and Human,Mouse

Overview

Product Description Mouse Monoclonal antibody [PIN.1] recognizes CD74
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC, IP, WB
Specificity Detects ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
Host Mouse
Clonality Monoclonal
Clone PIN.1
Isotype IgG
Target Name CD74
Antigen Species Human
Immunogen Human CD74 invariant chain synthetic peptide
Conjugation Un-conjugated
Alternate Names DHLAG; Ii; CD antigen CD74; II; HLA-DR antigens-associated invariant chain; Ia-GAMMA; Ia antigen-associated invariant chain; HLA class II histocompatibility antigen gamma chain; p33; HLADG

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1:50
IHC1:100
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16149 Mouse CD74

GeneID: 972 Human CD74

Swiss-port # P04233 Human HLA class II histocompatibility antigen gamma chain

Swiss-port # P04441 Mouse H-2 class II histocompatibility antigen gamma chain

Gene Symbol CD74
Gene Full Name CD74 molecule, major histocompatibility complex, class II invariant chain
Background The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Function Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. [UniProt]
Cellular Localization Cell membrane, Endoplasmic Reticulum, Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Lysosome
Calculated MW 34 kDa
PTM N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.