ARG57668

anti-CD75 / ST6GAL1 antibody

anti-CD75 / ST6GAL1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

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Overview

Product Description Rabbit Polyclonal antibody recognizes CD75 / ST6GAL1
Tested Reactivity Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD75 / ST6GAL1
Antigen Species Human
Immunogen Recombinant protein of Human CD75 / ST6GAL1.
Conjugation Un-conjugated
Alternate Names SIAT1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; B-cell antigen CD75; Sialyltransferase 1; ST6Gal I; Beta-galactoside alpha-2,6-sialyltransferase 1; EC 2.4.99.1; ST6N; Alpha 2,6-ST 1; ST6GalI

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse lung

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20440 Mouse ST6GAL1

Swiss-port # Q64685 Mouse Beta-galactoside alpha-2,6-sialyltransferase 1

Gene Symbol ST6GAL1
Gene Full Name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Background This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009]
Function Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates. [UniProt]
Calculated MW 47 kDa
PTM The soluble form derives from the membrane form by proteolytic processing.

The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. [UniProt]

Customer's Feedback

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Review for anti-CD75 / ST6GAL1 antibody

Application:WB

Sample:Mouse hybridoma

Sample Loading Amount:50 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC