ARG62427
anti-CD79a antibody [HM47/A9]
anti-CD79a antibody [HM47/A9] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Bovine,Human,Monkey,Mouse,Pig,Rat
Cancer antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [HM47/A9] recognizes CD79a |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Mk, Pig |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody labels precursor B-cell acute lymphoblastic leukemia samples, making it the most reliable B-cell marker for this disorder. In a study by Mason, et al on a total of 454 paraffin-embedded tissues, it reacted with 97% of B-cell neoplasms. We have data to indicate that this antibody may not cross react with Opossum. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HM47/A9 |
| Isotype | IgG1 |
| Target Name | CD79a |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (GTYQDVGSLNIADVQ), corresponding to C terminal amino acids 208-222 of Human CD79a |
| Conjugation | Un-conjugated |
| Alternate Names | Surface IgM-associated protein; B-cell antigen receptor complex-associated protein alpha chain; Membrane-bound immunoglobulin-associated protein; Ig-alpha; MB-1 membrane glycoprotein; MB-1; IGA; CD antigen CD79a |
Application Instructions
| Application Suggestion |
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| Application Note | IHC: 0.5 - 1 µg/ml FACS: 0.01µg for 106 cells WB: 0.5 - 1 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD79A |
| Gene Full Name | CD79a molecule, immunoglobulin-associated alpha |
| Background | CD79a: The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Function | CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells. [UniProt] |
| Highlight | Related products: CD79a antibodies; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 25 kDa |
| PTM | Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylated by LYN. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation. Arginine methylation in the ITAM domain may interfere with the binding of SYK. It promotes signals leading to B-cell differentiation (By similarity). |
Clone References
Comparative local immunogenic potential of scaffolds prepared from porcine cholecyst, jejunum, and urinary bladder in rat subcutaneous model.
B2 cells suppress experimental abdominal aortic aneurysms.
Blastic plasmacytoid dendritic cell neoplasm with leukemic presentation: an Italian multicenter study.
IHC / Human
POZ-, AT-hook-, and zinc finger-containing protein (PATZ) interacts with human oncogene B cell lymphoma 6 (BCL6) and is required for its negative autoregulation.
IHC-P /
Variation in characteristics of islets of Langerhans in insulin-resistant, diabetic and non-diabetic-rat strains.
IHC-P / Rat
A tumor-suppressor function for NFATc3 in T-cell lymphomagenesis by murine leukemia virus.
IHC / Mouse
Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
