ARG62926

anti-CD79a antibody [HM57]

anti-CD79a antibody [HM57] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat,Bovine,Chicken,Guinea pig,Horse,Opossum,Pig,Rabbit

Cancer antibody; Developmental Biology antibody; Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [HM57] recognizes CD79a

Tested Reactivity Hu, Ms, Rat, Bov, Chk, Gpig, Hrs, Opossum, Pig, Rb
Tested Application FACS, IHC-Fr, IHC-P
Specificity The clone HM57 interacts with CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
HLDA V; WS Code BC cB018
HLDA VI; WS Code BP 193
HLDA VI; WS Code BP 89
HLDA VI; WS Code B B103
HLDA VI; WS Code B CD79.4
Host Mouse
Clonality Monoclonal
Clone HM57
Isotype IgG1
Target Name CD79a
Antigen Species Human
Immunogen Synthetic peptide corresponding to amino acids 202-216 of human CD79a_x000D_
Conjugation Un-conjugated
Alternate Names Surface IgM-associated protein; B-cell antigen receptor complex-associated protein alpha chain; Membrane-bound immunoglobulin-associated protein; Ig-alpha; MB-1 membrane glycoprotein; MB-1; IGA; CD antigen CD79a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS5 µg/ml
IHC-Fr10 µg/ml
IHC-P10 µg/ml
Application Note FACS: Application note: Intracellular staining.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12518 Mouse CD79A

GeneID: 281674 Bovine CD79A

GeneID: 973 Human CD79A

Gene Symbol CD79A
Gene Full Name CD79a molecule, immunoglobulin-associated alpha
Background CD79a: The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Function CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells. [UniProt]
Highlight Related products:
CD79a antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 25 kDa
PTM Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylated by LYN. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
Arginine methylation in the ITAM domain may interfere with the binding of SYK. It promotes signals leading to B-cell differentiation (By similarity).