ARG41762

anti-CD79b antibody [CDLA79b-1]

anti-CD79b antibody [CDLA79b-1] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Mouse Monoclonal antibody [CDLA79b-1] recognizes CD79b
Tested Reactivity Hu
Tested Application IHC-P
Host Mouse
Clonality Monoclonal
Clone CDLA79b-1
Isotype IgG2b, kappa
Target Name CD79b
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 29-159 of Human CD79b.
Conjugation Un-conjugated
Alternate Names B29; B-cell-specific glycoprotein B29; Ig-beta; B-cell antigen receptor complex-associated protein beta chain; Immunoglobulin-associated B29 protein; AGM6; IGB; CD antigen CD79b

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 974 Human CD79B

Swiss-port # P40259 Human B-cell antigen receptor complex-associated protein beta chain

Gene Symbol CD79B
Gene Full Name CD79b molecule, immunoglobulin-associated beta
Background The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Function Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Note=Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. [UniProt]
Calculated MW 26 kDa
PTM Phosphorylated on tyrosine upon B-cell activation by SRC-type Tyr-kinases such as BLK, LYN and SYK. [UniProt]