ARG23411
anti-CD80 antibody [RFD1]
anti-CD80 antibody [RFD1] for IHC-Frozen sections and Human,Macaque
Overview
| Product Description | Mouse Monoclonal antibody [RFD1] recognizes CD80 Mouse anti Human dendritic cells antibody, clone RFD1 recognizes an epitope within the class II MHC complex. In normal tissue mouse anti human dendritic cells antibody, clone RFD1 identifies dendritic cells and a proportion of B lymphocytes. Within the macrophage/dendritic cell populations the epitope seen by RFD1 is co-expressed with RFD7 by a subset of cells that exhibit suppressive activity on T cell stimulation.Mouse anti Human dendritic cells antibody, clone RFD1 does not bind to monocytes or cells of the granulocyte lineage.Mouse anti Human dendritic cells antibody, clone RFD1 has been used for the induction of apoptosis via MHC Class II cross linking of gastric mucosa epithelial cells (Fan et al. 1998). |
|---|---|
| Tested Reactivity | Hu, Macaq |
| Tested Application | IHC-Fr |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | RFD1 |
| Isotype | IgM |
| Target Name | CD80 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | B7.1; CTLA-4 counter-receptor B7.1; CD28LG; T-lymphocyte activation antigen CD80; B7-1; CD28LG1; B7; LAB7; Activation B7-1 antigen; CD antigen CD80; BB1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P33681 Human T-lymphocyte activation antigen CD80 |
|---|---|
| Gene Symbol | CD80 |
| Gene Full Name | CD80 molecule |
| Background | The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011] |
| Function | Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. [UniProt] |
| Calculated MW | 33 kDa |
