ARG23243

anti-CD81 antibody [1D6]

anti-CD81 antibody [1D6] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Chimpanzee,Goat,Human,Sheep

Overview

Product Description Mouse Monoclonal antibody [1D6] recognizes CD81
Mouse anti Human CD81 antibody, clone 1D6 recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation. Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects.
Tested Reactivity Hu, Chimp, Goat, Sheep
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone 1D6
Isotype IgG1
Target Name CD81
Antigen Species Human
Immunogen OCI-LY8 cells.
Conjugation Un-conjugated
Alternate Names CD antigen CD81; TAPA1; Tspan-28; S5.7; CD81 antigen; Target of the antiproliferative antibody 1; Tetraspanin-28; 26 kDa cell surface protein TAPA-1; CVID6; TSPAN28

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:5 - 1:10
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 975 Human CD81

Swiss-port # P60033 Human CD81 antigen

Gene Symbol CD81
Gene Full Name CD81 molecule
Background The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Function May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. [UniProt]
Highlight Related products:
CD81 antibodies; Anti-Mouse IgG secondary antibodies;
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Calculated MW 26 kDa
PTM Not glycosylated. [UniProt]