ARG65623

anti-CD82 antibody [C33] (low endotoxin)

anti-CD82 antibody [C33] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Gene Regulation antibody; Immune System antibody

Overview

Product Description Low endotoxin Mouse Monoclonal antibody [C33] recognizes CD82 (low endotoxin)
Tested Reactivity Hu
Tested Application FACS, FuncSt, ICC/IF, IHC-P, IP, WB
Specificity This antibody reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
Host Mouse
Clonality Monoclonal
Clone C33
Isotype IgG2a
Target Name CD82
Antigen Species Human
Immunogen C91/PL (human HTLV-1+ T cell line)
Conjugation Un-conjugated
Alternate Names Tetraspanin-27; C33 antigen; R2; Tspan-27; TSPAN27; KAI1; CD antigen CD82; CD82 antigen; ST6; 4F9; Suppressor of tumorigenicity 6 protein; SAR2; IA4; Metastasis suppressor Kangai-1; Inducible membrane protein R2; GR15; C33

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note Functional application: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3732 Human CD82

Swiss-port # P27701 Human CD82 antigen

Gene Symbol CD82
Gene Full Name CD82 molecule
Background This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. [UniProt]
Research Area Cancer antibody; Gene Regulation antibody; Immune System antibody
Calculated MW 30 kDa