ARG65431
anti-CD84 antibody [CD84.1.21] (low endotoxin)
anti-CD84 antibody [CD84.1.21] (low endotoxin) for Flow cytometry,Immunoprecipitation,Functional study and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [CD84.1.21] recognizes CD84 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IP |
| Specificity | The clone CD84.1.21 recognizes CD84, a single chain cell surface glycoprotein of 64-82 kDa, predominantly expressed B cells, monocytes, platelets and some T cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CD84.1.21 |
| Isotype | IgG2a |
| Target Name | CD84 |
| Immunogen | CD84-transfected 300.19 cell line |
| Conjugation | Un-conjugated |
| Alternate Names | hCD84; Leukocyte differentiation antigen CD84; Hly9-beta; CD antigen CD84; LY9B; mCD84; Cell surface antigen MAX.3; SLAM family member 5; SLAMF5; Signaling lymphocytic activation molecule 5 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: Enhancement of CD3-induced IFN gamma production. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD84 |
| Gene Full Name | CD84 molecule |
| Background | CD84 is a highly glycosylated homophilic receptor of SLAM family. It is expressed on platelets and various types of leukocytes, especially following their activation. Ligation of CD84 leads to its phosphorylation on tyrosine residues within the cytoplasmic tail. These docking sites are recognized by downstream signaling molecules, such as phosphatase SHP-2 and adaptor protein SAP/SH2D1A. The function of CD84 has not been fully elucidated yet. Although predominantly activating receptor, its modulating activity was also demonstrated. |
| Function | Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 39 kDa |
| PTM | Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A is dependent of tyrosine phosphorylation of its cytoplasmic domain. Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation. Phosphorylated on tyrosine residues upon high affinity immunoglobulin epsilon receptor aggregation in mast cells. N-glycosylated. |
Clone References
CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse.
CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1.
Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP.
