ARG65431

anti-CD84 antibody [CD84.1.21] (low endotoxin)

anti-CD84 antibody [CD84.1.21] (low endotoxin) for Flow cytometry,Immunoprecipitation,Functional study and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [CD84.1.21] recognizes CD84
Tested Reactivity Hu
Tested Application FACS, FuncSt, IP
Specificity The clone CD84.1.21 recognizes CD84, a single chain cell surface glycoprotein of 64-82 kDa, predominantly expressed B cells, monocytes, platelets and some T cells.
Host Mouse
Clonality Monoclonal
Clone CD84.1.21
Isotype IgG2a
Target Name CD84
Immunogen CD84-transfected 300.19 cell line
Conjugation Un-conjugated
Alternate Names hCD84; Leukocyte differentiation antigen CD84; Hly9-beta; CD antigen CD84; LY9B; mCD84; Cell surface antigen MAX.3; SLAM family member 5; SLAMF5; Signaling lymphocytic activation molecule 5

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncStAssay-dependent
IPAssay-dependent
Application Note Functional studies: Enhancement of CD3-induced IFN gamma production.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8832 Human CD84

Swiss-port # Q9UIB8 Human SLAM family member 5

Gene Symbol CD84
Gene Full Name CD84 molecule
Background CD84 is a highly glycosylated homophilic receptor of SLAM family. It is expressed on platelets and various types of leukocytes, especially following their activation. Ligation of CD84 leads to its phosphorylation on tyrosine residues within the cytoplasmic tail. These docking sites are recognized by downstream signaling molecules, such as phosphatase SHP-2 and adaptor protein SAP/SH2D1A. The function of CD84 has not been fully elucidated yet. Although predominantly activating receptor, its modulating activity was also demonstrated.
Function Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 39 kDa
PTM Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A is dependent of tyrosine phosphorylation of its cytoplasmic domain. Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation. Phosphorylated on tyrosine residues upon high affinity immunoglobulin epsilon receptor aggregation in mast cells.
N-glycosylated.

Clone References

CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse.

Romero X et al.
J Immunol.,  (2005)

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CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1.

Martin M et al.
J Immunol.,  (2001)

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Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP.

Sayós J et al.
Blood.,  (2001)

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