ARG43320

anti-CD85d / ILT4 antibody

anti-CD85d / ILT4 antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CD85d / ILT4
Tested Reactivity Ms, Rat
Predict Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD85d / ILT4
Antigen Species Human
Immunogen Recombinant protein within aa. 61-300 of Human CD85d / ILT4.
Conjugation Un-conjugated
Alternate Names ILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000 - 1:4000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse blood cells
Observed Size ~ 65 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol LILRB2
Gene Full Name leukocyte immunoglobulin like receptor B2
Background This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:20448110, PubMed:27859042, PubMed:16455647). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885). [UniProt]
Calculated MW 65 kDa