ARG23623

anti-CD85j / LIR1 antibody [4F9]

anti-CD85j / LIR1 antibody [4F9] for IHC-Frozen sections,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [4F9] recognizes CD85j / LIR1
Tested Reactivity Hu
Tested Application FACS, IHC-Fr
Host Mouse
Clonality Monoclonal
Clone 4F9
Isotype IgG1
Target Name CD85j / LIR1
Antigen Species Human
Immunogen Monocyte derived dendritic cells.
Conjugation Un-conjugated
Alternate Names LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT2; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; LIR1; ILT-2; Leukocyte immunoglobulin-like receptor subfamily B member 1; Monocyte/macrophage immunoglobulin-like receptor 7; CD antigen CD85j; CD85J; MIR7; MIR-7

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:5
IHC-FrAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10859 Human LILRB1

Swiss-port # Q8NHL6 Human Leukocyte immunoglobulin-like receptor subfamily B member 1

Gene Symbol LILRB1
Gene Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Background This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. [UniProt]
Calculated MW 71 kDa
PTM Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. [UniProt]