ARG21089
anti-CD86 antibody [2D10] (FITC)
anti-CD86 antibody [2D10] (FITC) for Flow cytometry,IHC-Frozen sections,Blocking,ELISA and Mouse
Publication1
Overview
| Product Description | FITC-conjugated Rat Monoclonal antibody [2D10] recognizes CD86 |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | BL, ELISA, FACS, IHC-Fr |
| Specificity | Mouse CD86. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 2D10 |
| Isotype | IgG2b, kappa |
| Target Name | CD86 |
| Antigen Species | Mouse |
| Immunogen | Mouse B cell lymphoma cell line 5C2 |
| Conjugation | FITC |
| Alternate Names | B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P42082 Mouse T-lymphocyte activation antigen CD86 |
|---|---|
| Gene Symbol | CD86 |
| Gene Full Name | CD86 antigen |
| Background | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt] |
| Calculated MW | 38 kDa |
| PTM | Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
Specific References
Holothurian triterpene glycoside cucumarioside A2-2 induces macrophages activation and polarization in cancer immunotherapy
FACS / Mouse
