ARG62936

anti-CD86 antibody [GL-1] (FITC)

anti-CD86 antibody [GL-1] (FITC) for Blocking,Flow cytometry,IHC-Frozen sections and Mouse

Developmental Biology antibody; Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description

FITC-conjugated Rat Monoclonal antibody [GL-1] recognizes CD86

Tested Reactivity Ms
Tested Application BL, FACS, IHC-Fr
Host Rat
Clonality Monoclonal
Clone GL-1
Isotype IgG2a, kappa
Target Name CD86
Antigen Species Mouse
Immunogen LPS-activated CBA/Ca mouse spleen B cells
Conjugation FITC
Alternate Names B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12524 Mouse CD86

Swiss-port # P42082 Mouse T-lymphocyte activation antigen CD86

Gene Symbol Cd86
Gene Full Name CD86 antigen
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 38 kDa
PTM Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Clone References

Inflammatory cascades mediate synapse elimination in spinal cord compression.

IHC-FoFr / Mouse

Takano M et al.
J Neuroinflammation.,  (2014)

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Live single cell functional phenotyping in droplet nano-liter reactors.

Konry T et al.
Sci Rep.,  (2013)

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Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells.

Edgtton KL et al.
J Am Soc Nephrol.,  (2008)

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Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells.

Steptoe RJ et al.
Diabetes.,  (2005)

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Incomplete activation of CD4 T cells by antigen-presenting transitional immature B cells: implications for peripheral B and T cell responsiveness.

Chung JB et al.
J Immunol.,  (2003)

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