ARG23267

anti-CD86 antibody [IL-A190] (FITC)

anti-CD86 antibody [IL-A190] (FITC) for Flow cytometry and Bovine,Sheep

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [IL-A190] recognizes CD86
Clone IL-A190 recognises the bovine CD86 cell surface antigen, which is expressed by dendritic cells, activated macrophages and activated B cells. CD86 plays an important role in co-stimulation of T cells in the primary immune response.
Tested Reactivity Bov, Sheep
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone IL-A190
Isotype IgG1
Target Name CD86
Antigen Species Bovine
Conjugation FITC
Alternate Names B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25 - 1:200
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide and 1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD86
Gene Full Name CD86 molecule
Background This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
Calculated MW 38 kDa
PTM Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. [UniProt]