ARG22846
anti-CD88 / C5AR1 antibody [P12/1]
anti-CD88 / C5AR1 antibody [P12/1] for IHC-Frozen sections,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rhesus Monkey
Overview
| Product Description | Mouse Monoclonal antibody [P12/1] recognizes CD88 / C5AR1 |
|---|---|
| Tested Reactivity | Hu, R. Mk |
| Tested Application | FACS, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | P12/1 |
| Isotype | IgG2a |
| Target Name | CD88 / C5AR1 |
| Antigen Species | Human |
| Immunogen | C5aR - peptide: Met1 - Asn31 |
| Conjugation | Un-conjugated |
| Alternate Names | CD88; C5R1; C5AR; CD antigen CD88; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 5 x 10^5 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P21730 Human C5a anaphylatoxin chemotactic receptor 1 |
|---|---|
| Gene Symbol | C5AR1 |
| Gene Full Name | complement component 5a receptor 1 |
| Function | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. [UniProt] |
| Calculated MW | 39 kDa |
| PTM | Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist (PubMed:11342590). Sulfation at Tyr-14 is important for CHIPS binding (PubMed:21706042). Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338. |
