ARG22519

anti-CD89 antibody [MIP8a] (low endotoxin)

anti-CD89 antibody [MIP8a] (low endotoxin) for ELISA,Flow cytometry,Functional study,Immunoprecipitation,Western blot and Human

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [MIP8a] recognizes CD89
This antibody recognizes the human CD89 cell surface antigen, a 50-75kDa cell surface glycoprotein that is also known as the IgA receptor (Fc alpha R).CD89 is expressed by peripheral blood monocytes and neutrophils.MIP8a blocks binding of IgA to the Fc alpha R, and also inhibits neutrophil phagocytosis of IgA complexes.
Tested Reactivity Hu
Tested Application ELISA, FACS, FuncSt, IP, WB
Host Mouse
Clonality Monoclonal
Clone MIP8a
Isotype IgG1
Target Name CD89
Antigen Species Human
Immunogen Recombinant soluble human Fc alpha R.
Conjugation Un-conjugated
Alternate Names CD89; CTB-61M7.2; FcalphaRI; CD antigen CD89; Immunoglobulin alpha Fc receptor; IgA Fc receptor

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1:50 - 1:100
FuncStAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells or 100ul whole blood
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Purification Note Low endotoxin
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2204 Human FCAR

Swiss-port # P24071 Human Immunoglobulin alpha Fc receptor

Gene Symbol FCAR
Gene Full Name Fc fragment of IgA receptor
Background This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Function Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production. [UniProt]
Calculated MW 32 kDa