ARG65386

anti-CD8b antibody [341]

anti-CD8b antibody [341] for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Rat

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [341] recognizes CD8b
Tested Reactivity Rat
Tested Application FACS, FuncSt, IHC-Fr, IP, WB
Specificity The clone 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor, expressed on T cell subsets and some other cell types, such as macrophages.
Host Mouse
Clonality Monoclonal
Clone 341
Isotype IgG1
Target Name CD8b
Immunogen CD8 positive Wistar rat splenic T cell hybridomas
Conjugation Un-conjugated
Alternate Names LY3; CD8B1; CD antigen CD8b; LEU2; T-cell surface glycoprotein CD8 beta chain; P37; LYT3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1.5 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note Functional studies: Macrophage stimulation, in vitro blocking of allogenic responses.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24931 Rat CD8B

Swiss-port # P05541 Rat T-cell surface glycoprotein CD8 beta chain

Gene Symbol Cd8b
Gene Full Name CD8b molecule
Background The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. [UniProt]
Highlight Related products:
CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Immune System antibody
Calculated MW 24 kDa
PTM Phosphorylated as a consequence of T-cell activation.

Clone References

Rapid recovery from T lymphopenia by CD28 superagonist therapy.

Elflein K et al.
Blood.,  (2003)

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Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major.

Hirji N et al.
J Immunol.,  (1998)

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