ARG65386
anti-CD8b antibody [341]
anti-CD8b antibody [341] for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Rat
Immune System antibody
Overview
Product Description | Mouse Monoclonal antibody [341] recognizes CD8b |
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Tested Reactivity | Rat |
Tested Application | FACS, FuncSt, IHC-Fr, IP, WB |
Specificity | The clone 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor, expressed on T cell subsets and some other cell types, such as macrophages. |
Host | Mouse |
Clonality | Monoclonal |
Clone | 341 |
Isotype | IgG1 |
Target Name | CD8b |
Immunogen | CD8 positive Wistar rat splenic T cell hybridomas |
Conjugation | Un-conjugated |
Alternate Names | LY3; CD8B1; CD antigen CD8b; LEU2; T-cell surface glycoprotein CD8 beta chain; P37; LYT3 |
Application Instructions
Application Suggestion |
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Application Note | Functional studies: Macrophage stimulation, in vitro blocking of allogenic responses. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
Preservative | 15 mM Sodium azide |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # P05541 Rat T-cell surface glycoprotein CD8 beta chain |
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Gene Symbol | Cd8b |
Gene Full Name | CD8b molecule |
Background | The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. |
Function | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. [UniProt] |
Highlight | Related products: CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
Research Area | Immune System antibody |
Calculated MW | 24 kDa |
PTM | Phosphorylated as a consequence of T-cell activation. |