ARG22727

anti-CD8b antibody [CA15.4G2]

anti-CD8b antibody [CA15.4G2] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation and Dog

Overview

Product Description Mouse Monoclonal antibody [CA15.4G2] recognizes CD8b
This antibody recognizes Canine CD8β cell surface antigen, expressed by cytotoxic\suppressor T lymphocytes that interact with MHC Class I expressing targets.
Tested Reactivity Dog
Tested Application FACS, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone CA15.4G2
Isotype IgG1
Target Name CD8b
Antigen Species Dog
Conjugation Un-conjugated
Alternate Names LY3; CD8B1; CD antigen CD8b; LEU2; T-cell surface glycoprotein CD8 beta chain; P37; LYT3

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
IHC-Fr1:10
IPAssay-dependent
Application Note FACS: Use 20 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Unpurified.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD8B
Gene Full Name CD8b molecule
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]
Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. [UniProt]
Highlight Related products:
CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Tumor-Infiltrating Lymphocytes (TILs)
Calculated MW 24 kDa
PTM Phosphorylated as a consequence of T-cell activation.