ARG62945

anti-CD9 antibody [IVA50]

anti-CD9 antibody [IVA50] for Flow cytometry,Functional study,Immunoprecipitation,Western blot and Bovine

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [IVA50] recognizes CD9
Tested Reactivity Bov
Tested Application FACS, FuncSt, IP, WB
Specificity The clone IVA50 reacts with CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
Host Mouse
Clonality Monoclonal
Clone IVA50
Isotype IgG2
Target Name CD9
Immunogen Bovine thrombocytes.
Conjugation Un-conjugated
Alternate Names Leukocyte antigen MIC3; BTCC-1; TSPAN-29; MIC3; Tetraspanin-29; p24; Cell growth-inhibiting gene 2 protein; CD9 antigen; MRP-1; DRAP-27; 5H9 antigen; CD antigen CD9; TSPAN29; Motility-related protein; Tspan-29

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
FuncStAssay-dependent
IPAssay-dependent
WB1 µg/ml
Application Note Functional studies: The clone IVA50 activates Bovine thrombocytes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 280746 Bovine CD9

Swiss-port # P30932 Bovine CD9 antigen

Gene Symbol CD9
Gene Full Name CD9 molecule
Background This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
Function Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 25 kDa
PTM Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.

Clone References

Exosomes from bone marrow dendritic cells pulsed with diphtheria toxoid preferentially induce type 1 antigen-specific IgG responses in naive recipients in the absence of free antigen.

ELISA / Cow

Colino J et al.
J Immunol.,  (2006)

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A set of monoclonal antibodies specific for bovine cell surface molecule CD9.

Tomásková J et al.
Folia Biol (Praha).,  (1999)

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Nomenclature and characterization of leukocyte differentiation antigens in ruminants.

Naessens J et al.
Immunol Today.,  (1997)

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