ARG65501
anti-CD9 antibody [MEM-61] (azide free)
anti-CD9 antibody [MEM-61] (azide free) for Flow cytometry,Western blot,IHC-Formalin-fixed paraffin-embedded sections,Functional study and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Azide free Mouse Monoclonal antibody [MEM-61] recognizes CD9 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IHC-P, WB |
| Specificity | The clone MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. HLDA VI; WS Code P P-15 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-61 |
| Isotype | IgG1 |
| Target Name | CD9 |
| Immunogen | Pre-B cell line NALM-6. |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte antigen MIC3; BTCC-1; TSPAN-29; MIC3; Tetraspanin-29; p24; Cell growth-inhibiting gene 2 protein; CD9 antigen; MRP-1; DRAP-27; 5H9 antigen; CD antigen CD9; TSPAN29; Motility-related protein; Tspan-29 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. Functional studies: The clone MEM-61 induces FcγR-dependent platelet aggregation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Prostate |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD9 |
| Gene Full Name | CD9 molecule |
| Background | CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors. |
| Function | Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 25 kDa |
| PTM | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. |
Clone References
A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration.
WB / Human
Hypoxia-induced changes in the bioactivity of cytotrophoblast-derived exosomes.
WB / Human
Sperm attachment and penetration competence in the human oocyte: a possible aetiology of fertilization failure involving the organization of oolemmal lipid raft microdomains influenced by the ΔΨm of subplasmalemmal mitochondria.
ICC/IF / Human
Role of interleukin 16 in multiple myeloma.
BL / Human
