ARG65399

anti-CD90 / Thy 1 antibody [5E10] (azide free)

anti-CD90 / Thy 1 antibody [5E10] (azide free) for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot,CyTOF®-candidate and Human,Primates,Pig,Dog

Developmental Biology antibody; Immune System antibody; Neuroscience antibody

Overview

Product Description Azide free Mouse Monoclonal antibody [5E10] recognizes CD90 / Thy 1
Tested Reactivity Hu, Dog, NHuPrm, Pig
Tested Application CyTOF®-candidate, FACS, ICC/IF, IHC-Fr, IP, WB
Specificity The clone 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons.
Host Mouse
Clonality Monoclonal
Clone 5E10
Isotype IgG1
Target Name CD90 / Thy 1
Immunogen HEL erythroleukemia cells.
Conjugation Un-conjugated
Alternate Names Thy-1 membrane glycoprotein; Thy-1 antigen; CD antigen CD90; CDw90; CD90

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 3 µg/ml
ICC/IFAssay-dependent
IHC-Fr5 - 10 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note Preserved by filter sterilization.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7070 Human THY1

Swiss-port # P04216 Human Thy-1 membrane glycoprotein

Gene Symbol THY1
Gene Full Name Thy-1 cell surface antigen
Background CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.
Function May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. [UniProt]
Highlight Related products:
CD90 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody
Calculated MW 18 kDa

Clone References

Amniotic fluid-derived mesenchymal stem cells prevent fibrosis and preserve renal function in a preclinical porcine model of kidney transplantation.

Baulier E et al.
Stem Cells Transl Med.,  (2014)

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Immunopathogenic role of TH1 cells in autoimmune diabetes: evidence from a T1 and T2 doubly transgenic non-obese diabetic mouse model.

Hung JT et al.
J Autoimmun.,  (2005)

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Expression of Thy-1 on human hematopoietic progenitor cells.

Craig W et al.
J Exp Med.,  (1993)

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Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood.

Mayani H et al.
Blood.,  (1994)

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