ARG62949
anti-CD90.2 / Thy 1.2 antibody [1aG4/C5] (Functional Grade)
anti-CD90.2 / Thy 1.2 antibody [1aG4/C5] (Functional Grade) for Flow cytometry,ICC/IF,Western blot,Functional study and Mouse
Developmental Biology antibody; Immune System antibody; Neuroscience antibody
Overview
| Product Description | Functional Grade Mouse Monoclonal antibody [1aG4/C5] recognizes CD90.2 / Thy 1.2 |
|---|---|
| Tested Reactivity | Ms |
| Species Does Not React With | Hu, Rat |
| Tested Application | FACS, FuncSt, ICC/IF, WB |
| Specificity | The clone 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI linked glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1aG4/C5 |
| Isotype | IgG3 |
| Target Name | CD90.2 / Thy 1.2 |
| Immunogen | Thymocytes from C3H/Di mice (CD90.2 / Thy 1.2 positive). |
| Conjugation | Un-conjugated |
| Alternate Names | Thy-1 membrane glycoprotein; Thy-1 antigen; CD antigen CD90; CDw90; CD90 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: The bound of clone 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells. Other studies approve that the clone 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | Preserved by filter sterilization. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Thy1 |
| Gene Full Name | thymus cell antigen 1, theta |
| Background | CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions. |
| Function | May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody; Neuroscience antibody |
| Calculated MW | 18 kDa |
Clone References
Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies.
Monoclonal IgG3 (kappa) antibodies against murine Thy-1.2 antigen produced by murine hybridomas. Differences in the specificity of the antigen binding site and in the structure of the hinge region.
