ARG62949

anti-CD90.2 / Thy 1.2 antibody [1aG4/C5] (Functional Grade)

anti-CD90.2 / Thy 1.2 antibody [1aG4/C5] (Functional Grade) for Flow cytometry,ICC/IF,Western blot,Functional study and Mouse

Developmental Biology antibody; Immune System antibody; Neuroscience antibody

Overview

Product Description Functional Grade Mouse Monoclonal antibody [1aG4/C5] recognizes CD90.2 / Thy 1.2
Tested Reactivity Ms
Species Does Not React With Hu, Rat
Tested Application FACS, FuncSt, ICC/IF, WB
Specificity The clone 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI linked glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes.
Host Mouse
Clonality Monoclonal
Clone 1aG4/C5
Isotype IgG3
Target Name CD90.2 / Thy 1.2
Immunogen Thymocytes from C3H/Di mice (CD90.2 / Thy 1.2 positive).
Conjugation Un-conjugated
Alternate Names Thy-1 membrane glycoprotein; Thy-1 antigen; CD antigen CD90; CDw90; CD90

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
WBAssay-dependent
Application Note Functional studies: The bound of clone 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells. Other studies approve that the clone 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note Preserved by filter sterilization.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21838 Mouse THY1

Swiss-port # P01831 Mouse Thy-1 membrane glycoprotein

Gene Symbol Thy1
Gene Full Name thymus cell antigen 1, theta
Background CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.
Function May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody
Calculated MW 18 kDa

Clone References

Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies.

Dráber P et al.
J Immunogenet.,  (1980)

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Monoclonal IgG3 (kappa) antibodies against murine Thy-1.2 antigen produced by murine hybridomas. Differences in the specificity of the antigen binding site and in the structure of the hinge region.

Zikán J et al.
Folia Biol (Praha).,  (1982)

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