ARG23627
anti-CD92 / SLC44A1 antibody [VIM-15b] (PE)
anti-CD92 / SLC44A1 antibody [VIM-15b] (PE) for Flow cytometry and Human
Overview
Product Description | PE-conjugated Mouse Monoclonal antibody [VIM-15b] recognizes CD92 / SLC44A1. This product recognizes the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells. CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine. Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10 (Wille, S. et al.). Removal of Sodium Azide is recommended prior to use in functional assays. |
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Tested Reactivity | Hu |
Tested Application | FACS |
Host | Mouse |
Clonality | Monoclonal |
Clone | VIM-15b |
Isotype | IgG2b |
Target Name | CD92 / SLC44A1 |
Antigen Species | Human |
Immunogen | MV4-11 acute monocyte leukaemia cells. |
Conjugation | PE |
Alternate Names | CTL1; CD92; Choline transporter-like protein 1; CDw92; CDW92; CD antigen CD92; CHTL1; Solute carrier family 44 member 1 |
Application Instructions
Application Suggestion |
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Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purification with Protein G. |
Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
Preservative | 0.09% Sodium azide |
Stabilizer | 1% BSA and 5% Sucrose |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q8WWI5 Human Choline transporter-like protein 1 |
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Gene Symbol | SLC44A1 |
Gene Full Name | solute carrier family 44 (choline transporter), member 1 |
Function | Choline transporter. May be involved in membrane synthesis and myelin production. [UniProt] |
Calculated MW | 73 kDa |