ARG23627

anti-CD92 / SLC44A1 antibody [VIM-15b] (PE)

anti-CD92 / SLC44A1 antibody [VIM-15b] (PE) for Flow cytometry and Human

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [VIM-15b] recognizes CD92 / SLC44A1.
This product recognizes the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells. CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine. Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10 (Wille, S. et al.). Removal of Sodium Azide is recommended prior to use in functional assays.
Tested Reactivity Hu
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone VIM-15b
Isotype IgG2b
Target Name CD92 / SLC44A1
Antigen Species Human
Immunogen MV4-11 acute monocyte leukaemia cells.
Conjugation PE
Alternate Names CTL1; CD92; Choline transporter-like protein 1; CDw92; CDW92; CD antigen CD92; CHTL1; Solute carrier family 44 member 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:10
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA and 5% Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23446 Human SLC44A1

Swiss-port # Q8WWI5 Human Choline transporter-like protein 1

Gene Symbol SLC44A1
Gene Full Name solute carrier family 44 (choline transporter), member 1
Function Choline transporter. May be involved in membrane synthesis and myelin production. [UniProt]
Calculated MW 73 kDa