ARG56208
anti-CD95 / Fas antibody
anti-CD95 / Fas antibody for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD95 / Fas |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD95 / Fas |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around the N-terminus of Rat CD95. (YTDRKHYSDKCRRCAFCDEGHGL) |
| Conjugation | Un-conjugated |
| Alternate Names | CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 mins. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.025% Sodium azide and 2.5% BSA. |
| Preservative | 0.025% Sodium azide |
| Stabilizer | 2.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25446 Mouse Tumor necrosis factor receptor superfamily member 6 Swiss-port # Q63199 Rat Tumor necrosis factor receptor superfamily member 6 |
|---|---|
| Gene Symbol | Fas |
| Gene Full Name | Fas cell surface death receptor |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Calculated MW | 35 - 38 kDa (unmodified); 40 - 55 kDa (glycosylated) |
| PTM | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
